eclipse te300 inverted epifluorescence microscope  (Nikon)

Journal: Biomaterials Translational

Article Title: Three-dimensional biofabrication of nanosecond laser micromachined nanofibre meshes for tissue engineered scaffolds

doi: 10.12336/biomatertransl.2023.02.005

Figure Lengend Snippet: Morphological assessment of the perforated micromachined fibre mesh. (A, B) First cytotoxicity test of the 500 μm micromachined PLGA mesh. (A) Light Microscopy image of the micromachined array showing good circularity. (B) Live/dead image showing excellent cell viability and the holes clearly defined. (C–F) Light microscopy images of the 200 (C), 300 (D), 400 (E), and 500 (F) μm hole arrays, which show good circularity and even distribution. (G–J) Epifluorescent microscope images of live ADSCs on the micromachined fibre mesh with 200 (G), 300 (H), 400 (I), and 500 (J) μm hole arrays, with excellent cell viability seen across all samples, and ‘bridging’ of cells across. (K–M) Light microscope images of three selected micromachined electrospun PCL fibre mesh samples, all micromachined with the same setting but with minor variances as a result of the non-uniform thickness of the PCL nanofibre mesh. Scale bars: 500 μm. PCL: polycaprolactone; PLGA: polylactic-co-glycolic acid.

Article Snippet: For live/dead staining, the samples were stained with fluorescein and propidium iodide at day 21 to assess cell growth and viability, and then viewed under an inverted epifluorescent microscope (Nikon Eclipse TE300 Epifluorescent inverted microscope, Minato city, Tokyo, Japan).

Techniques: Light Microscopy, Microscopy

Journal: Biomaterials Translational

Article Title: Three-dimensional biofabrication of nanosecond laser micromachined nanofibre meshes for tissue engineered scaffolds

doi: 10.12336/biomatertransl.2023.02.005

Figure Lengend Snippet: Live/dead staining of ADSCs on the alginate and collagen stacks. (A–F) Inverted epifluorescent microscope images after live/dead staining of ADSCs on the alginate stacks. (A) Slice 1 (top slice) showing some good cell viability on day 1. (B) Slice 2 (middle slice) showing very limited cell presence indicating no cell mobility through the construct on day 1. (C) Slice 3 (bottom slice) showing no cells and thus no mobility through the cross-linked alginate on day 3. (D) Slice 1 (top slice) showing good cell viability with some stain intake by the alginate on day 7. (E) Slice 2 (middle slice) showing limited cell viability and thus mobility on day 7. (F) Slice 3 (bottom slice) showing no cell viability and thus no mobility through the alginate even on day 7. (G–L) inverted epifluorescent microscope images after live/dead staining of ADSCs on the collagen stacks. (G) Slice 1 (top slice) showing good cell viability on day 1. (H) Slice 2 (middle slice) showing good cell viability and therefore cells must be able to travel through on day 1. (I) Slice 3 (bottom slice) showing good cell viability and thus demonstrating the mobility of cells through the collagen and the micromachined fibre mesh even after 1 day. (J) Slice 1 (top slice) showing good cell viability on day 4. (K) Slice 2 (middle slice) showing good cell viability and therefore cells must be able to travel through on day 4. (L) Slice 3 (bottom slice) showing good cell viability and thus demonstrating the mobility of cells through the collagen and the micromachined fibre mesh on day 4. Scale bars: 500 μm. ADSC: adipose derived stem cell.

Article Snippet: For live/dead staining, the samples were stained with fluorescein and propidium iodide at day 21 to assess cell growth and viability, and then viewed under an inverted epifluorescent microscope (Nikon Eclipse TE300 Epifluorescent inverted microscope, Minato city, Tokyo, Japan).

Techniques: Staining, Microscopy, Construct, Derivative Assay